|
KMID : 0379120100380010048
|
|
Korean Journal of Mycology
2010 Volume.38 No. 1 p.48 ~ p.53
|
|
|
Agrobacterium-mediated transformation using gill tissue of Flammulina velutipes
|
|
Park Soon-Young
Peer Arend F. van
Jang Kab-Yeul
Shin Pyung-Gyun
Park Yun-Jung
Yoo Young-Bok
Park Ki-Moon
Kong Won-Sik
|
|
|
Abstract
|
|
|
|
Agrobacterium-mediated transformation was conducted in order to generate DNA insertional mutants of Flammulina velutipes. Agrobacterium tumefaciens AGL-1 harboring pBGgHg was transformed into gill tissues of Flammulina velutipes strain KACC42777. The transformants resistant on hygromycine () were confirmed by PCR. The targeted insertional sites were amplified by inverse PCR and sequenced. To find the phenotype variation of all generated transformants, bottle cultivation which followed by the standard cultivation protocol were conducted. Color variation was observed on the cultivated fruiting bodies. Furthermore, the transformant pool will be used as a good genetic resources for studying gene function.
|
|
|
KEYWORD
|
|
|
Agrobacterium-mediated transformation, Flammulina velutipes, Fruiting bodies, PCR analysis, Transformants
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|